CamKII-mtPyronicSF
(Plasmid #228906)

• Purpose: Expression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834
• Depositing Lab: Ghazaleh Ashrafi
• Publication: Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. doi: 10.1126/sciadv.adp7423. Epub 2024 Nov 15.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5132
• Total vector size (bp): 6737
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: pyronic SF
• Alt name: Pyruvate Optical Nano-Indicator from CECs Single-Fluorophore
• Species: Synthetic
• Insert Size (bp): 1605
• Promoter: CamKII
• Tag / Fusion Protein:
  • Cox8 targetting (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: ggatccacccgccaccccatgtccgtcctgacgc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://pubmed.ncbi.nlm.nih.gov/38562794/ for bioRxiv preprint.