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Addgene

pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine
(Plasmid #228902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Total vector size (bp) 7192
  • Vector type
    Mammalian Expression, AAV, Synthetic Biology
  • Selectable markers
    Carb

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hydroxycarboxylic acid receptor 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1080
  • Mutation
    R108K
  • GenBank ID
    NM_030701.3
  • Entrez Gene
    Hcar2 (a.k.a. Gpr109a, Gpr109b, HM74, Niacr1, PUMA-G, Pumag, mHM74b)
  • Promoter Chicken Beta Actin
  • Tags / Fusion Proteins
    • P2A mCitrine (C terminal on insert)
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine was a gift from Bryan Roth (Addgene plasmid # 228902 ; http://n2t.net/addgene:228902 ; RRID:Addgene_228902)

  • For your References section:

    Structure-guided design of a peripherally restricted chemogenetic system. Hye Jin Kang, Brian E. Krumm, Adrien Tassou, Matan Geron, Jeffrey F. DiBerto, Nicholas J. Kapolka, Ryan H. Gumpper, Kensuke Sakamoto, D. Dewran Kocak, Reid H.J. Olsen, Xi-Ping Huang, Shicheng Zhang, Karen L. Huang, Saheem A. Zaidi, MyV.T. Nguyen, Min Jeong Jo, Vsevolod Katritch, Jonathan F. Fay, Grégory Scherrer, Bryan L. Roth. Cell, 2024 10.1016/j.cell.2024.11.001
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