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Addgene

Luke-2
(Plasmid #228900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGAPDH
  • Backbone size w/o insert (bp) 5655
  • Total vector size (bp) 6501
  • Vector type
    Acanthamoeba expression vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Carbohydrate binding domain cbm49 protein
  • Alt name
    Uncharacterized protein
  • Species
    Acanthamoeba castellanii Neff
  • Mutation
    none
  • GenBank ID
    XM_004337618.1
  • Promoter Own
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Luke-2 was a gift from John Samuelson (Addgene plasmid # 228900 ; http://n2t.net/addgene:228900 ; RRID:Addgene_228900)

  • For your References section:

    Cellulose binding and the timing of expression influence protein targeting to the double-layered cyst wall of Acanthamoeba. Kanakapura Sundararaj B, Goyal M, Samuelson J. mSphere. 2024 Sep 25;9(9):e0046624. doi: 10.1128/msphere.00466-24. Epub 2024 Aug 13. 10.1128/msphere.00466-24 PubMed 39136454
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