pJEx411c_pelB-NbD6-Strep2
(Plasmid
#228897)
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PurposePeriplasmic Expresssion of Strep-tagged anti-Zika NbD6
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228897 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJEx411c
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Backbone manufacturerDNA 2.0 (modified)
- Backbone size w/o insert (bp) 2983
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsE.coli BL21-DE3 for expression
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesb0379
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Alt namepelB-NbD6-Strep2
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SpeciesSynthetic
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Insert Size (bp)484
- Promoter T7
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Tag
/ Fusion Protein
- Strep2 (C terminal on backbone)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7 fw
- 3′ sequencing primer pENTR-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJEx411c_pelB-NbD6-Strep2 was a gift from Stefan Arold (Addgene plasmid # 228897 ; http://n2t.net/addgene:228897 ; RRID:Addgene_228897) -
For your References section:
Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection. Yuli Peng, Atheer Alqatari, Fabian Kiessling, Dominik Renn, Raik Grünberg, Stefan T. Arold, and Magnus Rueping. ACS Synthetic Biology Article ASAP 10.1021/acssynbio.4c00819