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pET29b_GST-NbD6
(Plasmid #228896)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228896 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-29b
  • Backbone manufacturer
    Twist Bioscience
  • Backbone size w/o insert (bp) 5370
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E.coli SHuffle T7 strain for expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GST-tagged NbD6
  • Alt name
    GST-NbD6
  • Species
    Synthetic
  • Insert Size (bp)
    1065
  • Promoter T7
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b_GST-NbD6 was a gift from Magnus Rueping (Addgene plasmid # 228896 ; http://n2t.net/addgene:228896 ; RRID:Addgene_228896)

  • For your References section:

    Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection. Yuli Peng, Atheer Alqatari, Fabian Kiessling, Dominik Renn, Raik Grünberg, Stefan T. Arold, and Magnus Rueping. ACS Synthetic Biology Article ASAP 10.1021/acssynbio.4c00819
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