Lenti-Trono-EFS-Blast-P2A-TurboRFP
(Plasmid
#228893)
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Purpose(Empty Backbone) Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228893 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVTHM
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-Trono-EFS-Blast-P2A-TurboRFP was a gift from Francisco J Sánchez-Rivera (Addgene plasmid # 228893 ; http://n2t.net/addgene:228893 ; RRID:Addgene_228893) -
For your References section:
High-throughput evaluation of genetic variants with prime editing sensor libraries. Gould SI, Wuest AN, Dong K, Johnson GA, Hsu A, Narendra VK, Atwa O, Levine SS, Liu DR, Sanchez Rivera FJ. Nat Biotechnol. 2024 Mar 12. doi: 10.1038/s41587-024-02172-9. 10.1038/s41587-024-02172-9 PubMed 38472508