Lenti-PEAR-mCherry
(Plasmid
#228892)
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PurposeAll-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228892 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVTHM
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Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP-IRES-mScarlet
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-PEAR-mCherry was a gift from Francisco J Sánchez-Rivera (Addgene plasmid # 228892 ; http://n2t.net/addgene:228892 ; RRID:Addgene_228892) -
For your References section:
High-throughput evaluation of genetic variants with prime editing sensor libraries. Gould SI, Wuest AN, Dong K, Johnson GA, Hsu A, Narendra VK, Atwa O, Levine SS, Liu DR, Sanchez Rivera FJ. Nat Biotechnol. 2024 Mar 12. doi: 10.1038/s41587-024-02172-9. 10.1038/s41587-024-02172-9 PubMed 38472508