pET28A-Rab8A
(Plasmid
#228880)
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PurposeExpression of His-tagged Rab8A in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRAB8A
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SpeciesH. sapiens (human)
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Mutationresidues 1-6 and 177-207 missing
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Entrez GeneRAB8A (a.k.a. MEL, RAB8)
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Tag
/ Fusion Protein
- His tag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28A-Rab8A was a gift from Sebastian Mathea (Addgene plasmid # 228880 ; http://n2t.net/addgene:228880 ; RRID:Addgene_228880)