pFB-EPCP5
(Plasmid
#228879)
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PurposeExpression of Z-tagged LRRK2 KW using Baculoviruses
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228879 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFastBac1
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLeucine-rich repeat kinase 2
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SpeciesH. sapiens (human)
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Insert Size (bp)3854
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MutationAmino acids deleted 1-1846
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Entrez GeneLRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
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Tag
/ Fusion Protein
- His-Z-TEV (N terminal on backbone)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFB-EPCP5 was a gift from Sebastian Mathea (Addgene plasmid # 228879 ; http://n2t.net/addgene:228879 ; RRID:Addgene_228879)