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Addgene

pENTR-EGFP-TEADiv2
(Plasmid #228874)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TEADiv2
  • Alt name
    TEAD dominant-negative inhibitor GFP tagged
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1077
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ENTRforw
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ENTR vector for Gateway cloning of TEADiv2, GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal. Optimized by a D93E mutation in the YAP1 TBD with respect to original TEADi (Addgene #140144). As with other dominant negative proteins, good levels of expression are necessary for TEADi to block TEADs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-EGFP-TEADiv2 was a gift from Ramiro Iglesias-Bartolome (Addgene plasmid # 228874 ; http://n2t.net/addgene:228874 ; RRID:Addgene_228874)
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