pcDNA4-Flag-Timeless
(Plasmid #22887)

• Depositing Lab: Aziz Sancar
• Publication: Unsal-Kacmaz et al Mol Cell Biol. 2005 Apr . 25(8):3109-16.

Backbone

• Vector backbone: pcDNA4a/myc-is
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Timeless
• Alt name: Tim
• Species: H. sapiens (human)
• Insert Size (bp): 3620
• Mutation: deleted normal start codon, replaced with start codon before Flag tag
• Entrez Gene: TIMELESS (a.k.a. FASPS4, TIM, TIM1, hTIM)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry