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HR700_HA2TP53_Ex5
(Plasmid #228860)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228860 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK (HR700)
  • Backbone manufacturer
    System Biosciences
  • Backbone size (bp) 7402
  • Modifications to backbone
    Cloning of homology arm 1 ranging from exon 4 to intron 4 of human TP53 (chr17:7,675,788-7,676,168) using BsrGI and BsaI; Cloning of homology arm 2 ranging from intron 4 to intron 6 of human TP53 (chr17:7,674,377-7,675,787) with BbsI recognition sites flanking exon 5 (chr17:7,675,036 and 7,675,254) using MluI and SalI
  • Vector type
    Mammalian Expression, Cre/Lox, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.03.10.531074 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HR700_HA2TP53_Ex5 was a gift from Thorsten Stiewe (Addgene plasmid # 228860 ; http://n2t.net/addgene:228860 ; RRID:Addgene_228860)

  • For your References section:

    Deep CRISPR mutagenesis characterizes the functional diversity of TP53 mutations. Funk JS, Klimovich M, Drangenstein D, Pielhoop O, Hunold P, Borowek A, Noeparast M, Pavlakis E, Neumann M, Balourdas DI, Kochhan K, Merle N, Bullwinkel I, Wanzel M, Elmshauser S, Teply-Szymanski J, Nist A, Procida T, Bartkuhn M, Humpert K, Mernberger M, Savai R, Soussi T, Joerger AC, Stiewe T. Nat Genet. 2025 Jan;57(1):140-153. doi: 10.1038/s41588-024-02039-4. Epub 2025 Jan 7. 10.1038/s41588-024-02039-4 PubMed 39774325
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