pACEBac1_Strep-Psc-BICD2(1-400)
(Plasmid
#228830)
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PurposeInsect cell expression of N-terminal portion of BICD2 (aa 1-400)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228830 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACEBac1
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBICD2
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SpeciesM. musculus (mouse)
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Mutationaa 1-400
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Entrez GeneBicd2
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Tag
/ Fusion Protein
- Strep (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.04.11.589026 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACEBac1_Strep-Psc-BICD2(1-400) was a gift from Andrew Carter (Addgene plasmid # 228830 ; http://n2t.net/addgene:228830 ; RRID:Addgene_228830) -
For your References section:
The intracellular bacterium Orientia tsutsugamushi hijacks the adaptor protein BICD2 for dynein-based motility. Manigrasso G, Saharat K, Kullapanich C, Boulanger J, Morgan TE, Kramer H, Salje J, Carter AP. bioRxiv 2024.04.11.589026 10.1101/2024.04.11.589026