pRSVBneoT
(Plasmid
#22883)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22883 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM3zf(+)
- Backbone size w/o insert (bp) 5200
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEarly region SV40
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SpeciesSV40
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Insert Size (bp)2658
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site StuI (destroyed during cloning)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was made more than 20 years ago. The vector (pGEM3zf(+)) originated from Promega while the cassette (pMCneo(PolyA)) from Stratagene
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSVBneoT was a gift from James Pipas (Addgene plasmid # 22883 ; http://n2t.net/addgene:22883 ; RRID:Addgene_22883) -
For your References section:
The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain. Srinivasan A, McClellan AJ, Vartikar J, Marks I, Cantalupo P, Li Y, Whyte P, Rundell K, Brodsky JL, Pipas JM. Mol Cell Biol. 1997 Aug . 17(8):4761-73. PubMed 9234732
