EGFP_EHMT1B1-3
(Plasmid
#228811)
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PurposeExpression of mouse EHMT1 with deletion of aa 1185-1298
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228811 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4733
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEHMT1
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Alt nameG9a-like protein (GLP)
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SpeciesM. musculus (mouse)
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Insert Size (bp)3500
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Mutationdeletion of aa 1185-1298
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Entrez GeneEhmt1 (a.k.a. 9230102N17Rik, D330003E03, Eu-HMTase1, GLP, GLP1, KMT1D, mKIAA1876)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySubcloned from V5-EHMT1 plasmid (doi: 10.15252/embr.201643260)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.01.564969 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP_EHMT1B1-3 was a gift from Shravanti Rampalli (Addgene plasmid # 228811 ; http://n2t.net/addgene:228811 ; RRID:Addgene_228811) -
For your References section:
A cytoplasmic form of EHMT1N methylates viral proteins to enable inclusion body maturation and efficient viral replication. Biligiri KK, Sharma NR, Mohanty A, Sarkar DP, Vemula PK, Rampalli S.. PLOS Biology 22(11): e3002871. 10.1371/journal.pbio.3002871