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mCherry_EHMT1_full length
(Plasmid #228810)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228810 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry_C1
  • Backbone size w/o insert (bp) 4722
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EHMT1
  • Alt name
    G9a-like protein (GLP)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3894
  • Mutation
    See Depositor Comments
  • Entrez Gene
    Ehmt1 (a.k.a. 9230102N17Rik, D330003E03, Eu-HMTase1, GLP, GLP1, KMT1D, mKIAA1876)
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Subcloned from V5-EHMT1 plasmid (doi: 10.15252/embr.201643260)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E240R mutation in the EHMT1 insert should not affect basal functionality.

Please visit https://doi.org/10.1101/2023.11.01.564969 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry_EHMT1_full length was a gift from Shravanti Rampalli (Addgene plasmid # 228810 ; http://n2t.net/addgene:228810 ; RRID:Addgene_228810)

  • For your References section:

    A cytoplasmic form of EHMT1N methylates viral proteins to enable inclusion body maturation and efficient viral replication. Biligiri KK, Sharma NR, Mohanty A, Sarkar DP, Vemula PK, Rampalli S.. PLOS Biology 22(11): e3002871. 10.1371/journal.pbio.3002871
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