pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA
(Plasmid
#228788)
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PurposeEcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228788 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAcBac3
- Total vector size (bp) 15070
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Vector typeMammalian Expression ; Baculoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameEcTyrRS
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Alt nameE. coli tyrosyl synthetase
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Alt namepAAFRS
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SpeciesE. coli
Gene/Insert 2
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Gene/Insert nameEcYtR
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SpeciesE. coli
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MutationTAG suppressor
Gene/Insert 3
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Gene/Insert nameEGFP***
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Alt nameEGFP-3-TAA-40-TGA-152-TAG
Gene/Insert 4
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Gene/Insert nameTEV protease
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA was a gift from Abhishek Chatterjee (Addgene plasmid # 228788 ; http://n2t.net/addgene:228788 ; RRID:Addgene_228788) -
For your References section:
An Efficient Opal-Suppressor Tryptophanyl Pair Creates New Routes for Simultaneously Incorporating up to Three Distinct Noncanonical Amino Acids into Proteins in Mammalian Cells. Osgood AO, Zheng Y, Roy SJS, Biris N, Hussain M, Loynd C, Jewel D, Italia JS, Chatterjee A. Angew Chem Int Ed Engl. 2023 May 2;62(19):e202219269. doi: 10.1002/anie.202219269. Epub 2023 Apr 12. 10.1002/anie.202219269 PubMed 36905325