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pAcBac3-EcTrp-TGA-EGFP**
(Plasmid #228786)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 228786 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcBac3
  • Total vector size (bp) 13448
  • Vector type
    Mammalian Expression ; Baculoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    EcTrpRS
  • Alt name
    EcWRS-h14
  • Alt name
    E. coli tryptophanyl synthetase
  • Species
    E. coli

Gene/Insert 2

  • Gene/Insert name
    EcWtR
  • Species
    E. coli
  • Mutation
    TGA suppressor

Gene/Insert 3

  • Gene/Insert name
    EGFP**
  • Alt name
    EGFP-40-TAG-152-TGA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcBac3-EcTrp-TGA-EGFP** was a gift from Abhishek Chatterjee (Addgene plasmid # 228786 ; http://n2t.net/addgene:228786 ; RRID:Addgene_228786)

  • For your References section:

    An Efficient Opal-Suppressor Tryptophanyl Pair Creates New Routes for Simultaneously Incorporating up to Three Distinct Noncanonical Amino Acids into Proteins in Mammalian Cells. Osgood AO, Zheng Y, Roy SJS, Biris N, Hussain M, Loynd C, Jewel D, Italia JS, Chatterjee A. Angew Chem Int Ed Engl. 2023 May 2;62(19):e202219269. doi: 10.1002/anie.202219269. Epub 2023 Apr 12. 10.1002/anie.202219269 PubMed 36905325
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