PET-28a-YTH domain of YTHDF3
(Plasmid
#228777)
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PurposeProtein expression pladmid for YTH domain of YTHDF3 in E.Coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228777 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePET28A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameYTH domain of YTHDF3
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SpeciesH. sapiens (human)
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Entrez GeneYTHDF3 (a.k.a. DF3)
- Promoter T7
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Tag
/ Fusion Protein
- 6*His
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PET-28a-YTH domain of YTHDF3 was a gift from Huiqing Zhou (Addgene plasmid # 228777 ; http://n2t.net/addgene:228777 ; RRID:Addgene_228777) -
For your References section:
Discovery of a new inhibitor for YTH domain-containing m(6)A RNA readers. Wang CH, Zhou H. RSC Chem Biol. 2024 Jul 25;5(9):914-923. doi: 10.1039/d4cb00105b. eCollection 2024 Aug 28. 10.1039/d4cb00105b PubMed 39211476