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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtg13-interacting protein
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Alt nameATG101
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SpeciesH. sapiens (human)
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Insert Size (bp)657
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Entrez GeneATG101 (a.k.a. C12orf44)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-hAtg101 was a gift from Noboru Mizushima (Addgene plasmid # 22876 ; http://n2t.net/addgene:22876 ; RRID:Addgene_22876) -
For your References section:
Atg101, a novel mammalian autophagy protein interacting with Atg13. Hosokawa N, Sasaki T, Iemura S, Natsume T, Hara T, Mizushima N. Autophagy. 2009 Oct . 5(7):973-9. 10.4161/auto.5.7.9296 PubMed 19597335
Map uploaded by the depositor.
