Skip to main content
Addgene

pCI-neo-HA-hAtg13
(Plasmid #22874)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCI-neo-HA
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autophagy-related genes 13
  • Alt name
    Atg13
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1560
  • GenBank ID
    AB014552
  • Entrez Gene
    ATG13 (a.k.a. KIAA0652, PARATARG8)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo-HA-hAtg13 was a gift from Noboru Mizushima (Addgene plasmid # 22874 ; http://n2t.net/addgene:22874 ; RRID:Addgene_22874)

  • For your References section:

    Atg101, a novel mammalian autophagy protein interacting with Atg13. Hosokawa N, Sasaki T, Iemura S, Natsume T, Hara T, Mizushima N. Autophagy. 2009 Oct . 5(7):973-9. 10.4161/auto.5.7.9296 PubMed 19597335
Related items:
Commonly requested with: