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pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10
(Plasmid #228735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV with Tol2
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
  • Species
    Neisseria meningitidis
  • Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A D911G K929R E932K
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10 was a gift from Erik Sontheimer (Addgene plasmid # 228735 ; http://n2t.net/addgene:228735 ; RRID:Addgene_228735)

  • For your References section:

    Rational Design of Enhanced Nme2Cas9 and Nme2SmuCas9 Nucleases and Base Editors. Bamidele N, Ansodaria A, Chen Z, Cheng H, Panwala R, Jazbec E, Sontheimer EJ. bioRxiv November 07, 2024 10.1101/2024.10.30.620986
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