pEJS2699-CMV-Nme2Cas9-E932R
(Plasmid
#228720)
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PurposeExpresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV with Tol2
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Vector typeMammalian Expression, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNme2Cas9-E932R nuclease
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SpeciesNeisseria meningitidis
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MutationNme2-Cas9 E932R
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEJS2699-CMV-Nme2Cas9-E932R was a gift from Erik Sontheimer (Addgene plasmid # 228720 ; http://n2t.net/addgene:228720 ; RRID:Addgene_228720) -
For your References section:
Rational Design of Enhanced Nme2Cas9 and Nme2SmuCas9 Nucleases and Base Editors. Bamidele N, Ansodaria A, Chen Z, Cheng H, Panwala R, Jazbec E, Sontheimer EJ. bioRxiv November 07, 2024 10.1101/2024.10.30.620986