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Addgene

pEJS2698-CMV-Nme2Cas9-D56R
(Plasmid #228719)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228719 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV with Tol2
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nme2Cas9-D56R nuclease
  • Species
    Neisseria meningitidis
  • Mutation
    Nme2-Cas9 D56R
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEJS2698-CMV-Nme2Cas9-D56R was a gift from Erik Sontheimer (Addgene plasmid # 228719 ; http://n2t.net/addgene:228719 ; RRID:Addgene_228719)

  • For your References section:

    Rational Design of Enhanced Nme2Cas9 and Nme2SmuCas9 Nucleases and Base Editors. Bamidele N, Ansodaria A, Chen Z, Cheng H, Panwala R, Jazbec E, Sontheimer EJ. bioRxiv November 07, 2024 10.1101/2024.10.30.620986
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