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Addgene

pKJ0463
(Plasmid #228715)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228715 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sumo_epT7
  • Species
    other
  • Mutation
    T3M/G47A/E643G

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.17.604015 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKJ0463 was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 228715 ; http://n2t.net/addgene:228715 ; RRID:Addgene_228715)

  • For your References section:

    Rapid in silico directed evolution by a protein language model with EVOLVEpro. Jiang K, Yan Z, Di Bernardo M, Sgrizzi SR, Villiger L, Kayabolen A, Kim B, Carscadden JK, Hiraizumi M, Nishimasu H, Gootenberg JS, Abudayyeh OO. Science 0, eadr6006 10.1126/science.adr6006 PubMed 39071429
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