pKJ423
(Plasmid
#228708)
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PurposeMammalian expression of Anti-CD71 LC (light chain); to be used with pKJ455 (Plasmid 228707) to make the antibody
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Depositing Labs
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression, Affinity Reagent/ Antibody
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-CD71 LC
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.17.604015 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKJ423 was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 228708 ; http://n2t.net/addgene:228708 ; RRID:Addgene_228708) -
For your References section:
Rapid in silico directed evolution by a protein language model with EVOLVEpro. Jiang K, Yan Z, Di Bernardo M, Sgrizzi SR, Villiger L, Kayabolen A, Kim B, Carscadden JK, Hiraizumi M, Nishimasu H, Gootenberg JS, Abudayyeh OO. Science 0, eadr6006 10.1126/science.adr6006 PubMed 39071429