Skip to main content
Addgene

pIND-GLucZ
(Plasmid #228704)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 228704 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pINDUCER10-PheS
  • Backbone manufacturer
    Stephen Elledge
  • Total vector size (bp) 13147
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Gaussia luciferase
  • Alt name
    GLuc
  • Species
    Gaussia princeps
  • Insert Size (bp)
    558

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GAAGTTGGAACTGTGTTTGGTGG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    non-silencing shRNA
  • Alt name
    nsh
  • Species
    Synthetic
  • Insert Size (bp)
    110

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ATCAAAGAGATAGCAAGGTATTCAGTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains a T nucleotide deletion in the LoxP just upstream of the IRES. This deletion is not expected to impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIND-GLucZ was a gift from Thorsten Stiewe (Addgene plasmid # 228704 ; http://n2t.net/addgene:228704 ; RRID:Addgene_228704)

  • For your References section:

    Monitoring the dynamics of clonal tumour evolution in vivo using secreted luciferases. Charles JP, Fuchs J, Hefter M, Vischedyk JB, Kleint M, Vogiatzi F, Schafer JA, Nist A, Timofeev O, Wanzel M, Stiewe T. Nat Commun. 2014 Jun 3;5:3981. doi: 10.1038/ncomms4981. 10.1038/ncomms4981 PubMed 24889111
Related items: