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Addgene

pGLucIPZ
(Plasmid #228703)

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Item Catalog # Description Quantity Price (USD)
Plasmid 228703 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pGIPZ
  • Backbone manufacturer
    Open Biosystems
  • Total vector size (bp) 11267
  • Modifications to backbone
    Deletion of an EcoRI site in the Hygromycin resistance gene
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Gaussia luciferase
  • Alt name
    GLuc
  • Species
    Gaussia princeps
  • Insert Size (bp)
    558

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ATCAAAGAGATAGCAAGGTATTCAGTT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    non-silencing shRNA
  • Alt name
    nsh
  • Insert Size (bp)
    110

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGLucIPZ was a gift from Thorsten Stiewe (Addgene plasmid # 228703 ; http://n2t.net/addgene:228703 ; RRID:Addgene_228703)

  • For your References section:

    Monitoring the dynamics of clonal tumour evolution in vivo using secreted luciferases. Charles JP, Fuchs J, Hefter M, Vischedyk JB, Kleint M, Vogiatzi F, Schafer JA, Nist A, Timofeev O, Wanzel M, Stiewe T. Nat Commun. 2014 Jun 3;5:3981. doi: 10.1038/ncomms4981. 10.1038/ncomms4981 PubMed 24889111
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