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Addgene

pCI-neo-mAtg12
(Plasmid #22870)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autophagy-related genes 12
  • Alt name
    Atg12
  • Alt name
    Apg12
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    430
  • Entrez Gene
    Atg12 (a.k.a. 4931423H11Rik, A330058M13Rik, Apg12l, Atg12l)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo-mAtg12 was a gift from Noboru Mizushima (Addgene plasmid # 22870 ; http://n2t.net/addgene:22870 ; RRID:Addgene_22870)

  • For your References section:

    Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method. Mizushima N, Yoshimori T, Ohsumi Y. FEBS Lett. 2002 Dec 18. 532(3):450-4. 10.1016/S0014-5793(02)03739-0 PubMed 12482611
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