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Addgene

ZIKV isolate PRVABC59 NS3 helicase domain
(Plasmid #228664)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228664 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-6HIS-GST-TEV-GG
  • Backbone manufacturer
    Centre for Medicines Discovery
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zika virus NS3 helicase
  • Species
    Zika virus
  • Insert Size (bp)
    2028
  • GenBank ID
    AWH65849.1
  • Tag / Fusion Protein
    • His-GST-TEV (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZIKV isolate PRVABC59 NS3 helicase domain was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228664 ; http://n2t.net/addgene:228664 ; RRID:Addgene_228664)
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