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ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain
(Plasmid #228663)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228663 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-6HIS-GST-TEV-GG
  • Backbone manufacturer
    Centre for Medicines Discovery
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zika virus portion of NS2B protease coexpressed with NS3 protease
  • Species
    Zika virus
  • Insert Size (bp)
    855
  • GenBank ID
    AMK79467.1
  • Tag / Fusion Protein
    • His-GST-TEV (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Co-expression of two proteins using two separate ribosome binding sites controlled by the same promotor.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228663 ; http://n2t.net/addgene:228663 ; RRID:Addgene_228663)
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