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Addgene

WNV isolate WN NY 2000-crow3356 NS5 RdRp full length
(Plasmid #228662)

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Item Catalog # Description Quantity Price (USD)
Plasmid 228662 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pNIC-6HIS-SUMO-GG
  • Backbone manufacturer
    Centre for Medicines Discovery
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    West nile NS5 RdRp
  • Species
    West Nile virus
  • Insert Size (bp)
    2220
  • GenBank ID
    AAM81752.1
  • Tag / Fusion Protein
    • His-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WNV isolate WN NY 2000-crow3356 NS5 RdRp full length was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228662 ; http://n2t.net/addgene:228662 ; RRID:Addgene_228662)
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