Skip to main content
Addgene

WNV strain-NY99 NS2B-NS3 protease fusion with non cleavable linker with mutation K to A to reduce autoproteolysis
(Plasmid #228660)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 228660 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pNIC-NHStIIT
  • Backbone manufacturer
    Structural Genomics Consortium
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    West Nile portion of NS2B protease fused to NS3
  • Species
    West Nile virus
  • Insert Size (bp)
    900
  • Mutation
    H163A - catalytically inactive/West Nile Virus NS2B-NS3 fusion protein K to A mutation to reduce autoproteolysis
  • Tag / Fusion Protein
    • His-Twin Strep-Tev (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fusion of WNV NS2B and NS3 proteases

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WNV strain-NY99 NS2B-NS3 protease fusion with non cleavable linker with mutation K to A to reduce autoproteolysis was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228660 ; http://n2t.net/addgene:228660 ; RRID:Addgene_228660)
Related items: