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SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker
(Plasmid #228657)

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Item Catalog # Description Quantity Price (USD)
Plasmid 228657 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC-NHSTIIT-GG
  • Backbone manufacturer
    Centre for Medicines Discovery
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLEV portion of NS2B protease fused to NS3
  • Species
    St. Louis encephalitis virus
  • Insert Size (bp)
    864
  • GenBank ID
    YP_001008348.1
  • Tag / Fusion Protein
    • His-Twin Strep-Tev (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fusion of SLEV NS2B and NS3 proteases

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228657 ; http://n2t.net/addgene:228657 ; RRID:Addgene_228657)
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