pcDNA3-K27 histone methylation reporter
(Plasmid #22865)

• Depositing Lab: Alice Ting
• Publication: Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982-3.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: K27 HMR
• Species: E. Coli
• Insert Size (bp): 1772
• Tags / Fusion Proteins:
  • CFP (N terminal on insert)
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AflII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry