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Addgene

MERS-CoV Mpro strain HCoV-EMC 3CL protease domain
(Plasmid #228646)

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Item Catalog # Description Quantity Price (USD)
Plasmid 228646 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pNIC-6HIS-SUMO-GG
  • Backbone manufacturer
    Centre for Medicines Discovery
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MERS-Cov Mpro domain
  • Species
    MERS-CoV
  • Insert Size (bp)
    1239
  • Tag / Fusion Protein
    • His-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MERS-CoV Mpro strain HCoV-EMC 3CL protease domain was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228646 ; http://n2t.net/addgene:228646 ; RRID:Addgene_228646)
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