MERS-CoV Mpro strain HCoV-EMC 3CL protease domain
(Plasmid
#228646)
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PurposeBacterial expression of the MERS-CoV Mpro domain for biochemical assay.
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228646 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNIC-6HIS-SUMO-GG
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Backbone manufacturerCentre for Medicines Discovery
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMERS-Cov Mpro domain
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SpeciesMERS-CoV
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Insert Size (bp)1239
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Tag
/ Fusion Protein
- His-SUMO (N terminal on insert)
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MERS-CoV Mpro strain HCoV-EMC 3CL protease domain was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228646 ; http://n2t.net/addgene:228646 ; RRID:Addgene_228646)