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Addgene

SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain
(Plasmid #228643)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    Structural Genomics Consortium
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 NSP3 macrodomain
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    615
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • His-Tev (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228643 ; http://n2t.net/addgene:228643 ; RRID:Addgene_228643)
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