Coxsackievirus B4 (CVB4) 3C protease isolate CVB4/USA/9R1/2012
(Plasmid
#228640)
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PurposeBacterial expression of the Coxsackievirus 43 isoform B 3C protease
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepNIC-NOTAG-GG
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Backbone manufacturerCentre for Medicines Discovery
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCoxsackievirus 43 isoform B 3C protease
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SpeciesCoxsackievirus 43 isoform B
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Insert Size (bp)888
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GenBank IDWLJ60573.1
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Tag
/ Fusion Protein
- His-SUMO (N terminal on insert)
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Coxsackievirus B4 (CVB4) 3C protease isolate CVB4/USA/9R1/2012 was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228640 ; http://n2t.net/addgene:228640 ; RRID:Addgene_228640)