Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease
(Plasmid
#228637)
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PurposeBacterial expression of the Coxsackievirus 16 3C protease
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228637 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepNIC-NOTAG-GG
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Backbone manufacturerCentre for Medicines Discovery
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCoxsackievirus 16 3C protease
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SpeciesCoxsackievirus 16
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Insert Size (bp)888
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GenBank IDQWX10039.1
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Tag
/ Fusion Protein
- His-SUMO (N terminal on insert)
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228637 ; http://n2t.net/addgene:228637 ; RRID:Addgene_228637)
