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EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site
(Plasmid #228633)

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Item Catalog # Description Quantity Price (USD)
Plasmid 228633 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pSUMO-LIC
  • Backbone manufacturer
    Structural Genomics Consortium
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enterovirus Coxsackievirus A71 2A protease
  • Species
    Enterovirus A71
  • Insert Size (bp)
    465
  • Mutation
    C110A
  • GenBank ID
    JN544418.1
  • Tag / Fusion Protein
    • His-SUMO (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228633 ; http://n2t.net/addgene:228633 ; RRID:Addgene_228633)
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