EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site
(Plasmid
#228633)
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PurposeBacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228633 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSUMO-LIC
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Backbone manufacturerStructural Genomics Consortium
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEnterovirus Coxsackievirus A71 2A protease
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SpeciesEnterovirus A71
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Insert Size (bp)465
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MutationC110A
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GenBank IDJN544418.1
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Tag
/ Fusion Protein
- His-SUMO (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228633 ; http://n2t.net/addgene:228633 ; RRID:Addgene_228633)