Human Coxsackievirus A16 strain G10 2A protease
(Plasmid
#228632)
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PurposeBacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228632 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNIC-6HIS-SUMO-GG
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Backbone manufacturerCentre for Medicines Discovery
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEnterovirus Coxsackievirus A16 2A protease
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SpeciesCoxsackievirus 16
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Insert Size (bp)753
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GenBank IDNP_042242.1
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Entrez GeneHEVAgp1 (a.k.a. HEVAgp1)
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Tag
/ Fusion Protein
- His-SUMO (N terminal on backbone)
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human Coxsackievirus A16 strain G10 2A protease was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 228632 ; http://n2t.net/addgene:228632 ; RRID:Addgene_228632)