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pR-mOrange2
(Plasmid #228608)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pReceiver
  • Backbone manufacturer
    Birtwistle lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mOrange2
  • Species
    Synthetic
  • Insert Size (bp)
    708
  • Mutation
    We introduced some silent mutations to avoid essential restriction enzyme sites.
  • Promoter CMV

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.23.619855 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pR-mOrange2 was a gift from Marc Birtwistle (Addgene plasmid # 228608 ; http://n2t.net/addgene:228608 ; RRID:Addgene_228608)

  • For your References section:

    Genetically-Encoded Fluorescence Barcodes for Single-Cell Analysis. Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye F, Biswas T, Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR. bioRxiv [Preprint]. 2024 Oct 24:2024.10.23.619855. doi: 10.1101/2024.10.23.619855. 10.1101/2024.10.23.619855 PubMed 39484616
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