pR-mBeRFP
(Plasmid
#228601)
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PurposeMammalian expression of mBeRFP, for MuSIC barcode construction
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228601 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepReceiver
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Backbone manufacturerBirtwistle lab
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemBeRFP
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SpeciesSynthetic
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Insert Size (bp)705
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MutationWe introduced some silent mutations to avoid essential restriction enzyme sites.
- Promoter CMV
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.10.23.619855 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pR-mBeRFP was a gift from Marc Birtwistle (Addgene plasmid # 228601 ; http://n2t.net/addgene:228601 ; RRID:Addgene_228601) -
For your References section:
Genetically-Encoded Fluorescence Barcodes for Single-Cell Analysis. Lu X, Pritko DJ, Abravanel ME, Huggins JR, Ogunleye F, Biswas T, Ashy KC, Woods SK, Livingston MWT, Blenner MA, Birtwistle MR. bioRxiv [Preprint]. 2024 Oct 24:2024.10.23.619855. doi: 10.1101/2024.10.23.619855. 10.1101/2024.10.23.619855 PubMed 39484616