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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22860 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIRF3
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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Entrez GeneIRF3 (a.k.a. IIAE7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3962 pcDNA3-IRF3-FL was a gift from Peter Howley (Addgene plasmid # 22860 ; http://n2t.net/addgene:22860 ; RRID:Addgene_22860) -
For your References section:
Identification of TRAIL as an interferon regulatory factor 3 transcriptional target. Kirshner JR, Karpova AY, Kops M, Howley PM. J Virol. 2005 Jul . 79(14):9320-4. 10.1128/JVI.79.14.9320-9324.2005 PubMed 15994827