TMEM16F-I611K-peGFP-N1
(Plasmid
#228581)
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PurposeExpresses mouse TMEM16F-I611K-eGFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepeGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTMEM16F/anoctamin 6 (Ano6), transcript variant 2
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SpeciesM. musculus (mouse)
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MutationI611K plus a 3 amino acid N-terminal truncation (MQM)
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Entrez GeneAno6 (a.k.a. 2900059G15Rik, F730003B03Rik, Tmem16f)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.02.04.578431 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TMEM16F-I611K-peGFP-N1 was a gift from Huanghe Yang (Addgene plasmid # 228581 ; http://n2t.net/addgene:228581 ; RRID:Addgene_228581) -
For your References section:
TMEM16 and OSCA/TMEM63 proteins share a conserved potential to permeate ions and phospholipids. Lowry AJ, Liang P, Song M, Serena Wan YC, Pei ZM, Yang H, Zhang Y. bioRxiv [Preprint]. 2024 Sep 3:2024.02.04.578431. doi: 10.1101/2024.02.04.578431. 10.1101/2024.02.04.578431 PubMed 38370744