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TMEM16F-M522K-peGFP-N1
(Plasmid #228579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228579 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMEM16F/anoctamin 6 (Ano6), transcript variant 2
  • Species
    M. musculus (mouse)
  • Mutation
    M522K plus a 3 amino acid N-terminal truncation (MQM)
  • Entrez Gene
    Ano6 (a.k.a. 2900059G15Rik, F730003B03Rik, Tmem16f)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit  https://doi.org/10.1101/2024.02.04.578431 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TMEM16F-M522K-peGFP-N1 was a gift from Huanghe Yang (Addgene plasmid # 228579 ; http://n2t.net/addgene:228579 ; RRID:Addgene_228579)

  • For your References section:

    TMEM16 and OSCA/TMEM63 proteins share a conserved potential to permeate ions and phospholipids. Lowry AJ, Liang P, Song M, Serena Wan YC, Pei ZM, Yang H, Zhang Y. bioRxiv [Preprint]. 2024 Sep 3:2024.02.04.578431. doi: 10.1101/2024.02.04.578431. 10.1101/2024.02.04.578431 PubMed 38370744
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