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Addgene

pX330-U6-BRD4-HaloTag-Guide-hspCas9
(Plasmid #228576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330
  • Backbone manufacturer
    Feng Zhang (Addgene plasmid # 42230)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRD4 gRNA
  • gRNA/shRNA sequence
    CACCG GGGATCACTAGCATGTCTG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    24
  • Entrez Gene
    BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330-U6-BRD4-HaloTag-Guide-hspCas9 was a gift from Marcin Imielinski (Addgene plasmid # 228576 ; http://n2t.net/addgene:228576 ; RRID:Addgene_228576)
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