pUC57-Homology Arms-BRD4-HaloTag
(Plasmid
#228575)
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PurposeCRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertion
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228575 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHomology Arms-BRD4-HaloTag
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SpeciesH. sapiens (human)
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Insert Size (bp)1960
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Entrez GeneBRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57-Homology Arms-BRD4-HaloTag was a gift from Marcin Imielinski (Addgene plasmid # 228575 ; http://n2t.net/addgene:228575 ; RRID:Addgene_228575)
