AURKA-Aquamarine
(Plasmid
#228564)
-
PurposeEncodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoter
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 228564 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
AURKA-Aquamarine was a gift from Giulia Bertolin (Addgene plasmid # 228564 ; http://n2t.net/addgene:228564 ; RRID:Addgene_228564) -
For your References section:
tdLanYFP, a Yellow, Bright, Photostable, and pH-Insensitive Fluorescent Protein for Live-Cell Imaging and Forster Resonance Energy Transfer-Based Sensing Strategies. Bousmah Y, Valenta H, Bertolin G, Singh U, Nicolas V, Pasquier H, Tramier M, Merola F, Erard M. ACS Sens. 2021 Nov 26;6(11):3940-3947. doi: 10.1021/acssensors.1c00874. Epub 2021 Oct 22. 10.1021/acssensors.1c00874 PubMed 34676768