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Addgene

Aquamarine-LC3B
(Plasmid #228559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAP1LC3B
  • Alt name
    LC3B
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Promoter CMV
  • Tag / Fusion Protein
    • Aquamarine (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Aquamarine-LC3B was a gift from Giulia Bertolin (Addgene plasmid # 228559 ; http://n2t.net/addgene:228559 ; RRID:Addgene_228559)

  • For your References section:

    The LC3B FRET biosensor monitors the modes of action of ATG4B during autophagy in living cells. Gokerkucuk EB, Cheron A, Tramier M, Bertolin G. Autophagy. 2023 Aug;19(8):2275-2295. doi: 10.1080/15548627.2023.2179845. Epub 2023 Feb 22. 10.1080/15548627.2023.2179845 PubMed 36814061
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